I think those who are well-immersed in the scientific community are familiar with the biological databases connected through the website www.ncbi.nlm.nih.gov. I was just wondering if someone could possibly explain to me a little about the Blast database for me. IN a particular section of a homework assignment, Blast is needed to query for matching protein/gene sequences. The search for an unc. C elegan prtoein/gene sequence resulted in a bunch of data on species that have similar protein/gene sequences and alignments. An example for the protein sequence results are: Query 1 MEHEKDPGWQYLRRTREQVLEDQSKPYDSKKNVWIPDPEEGYLAGEITATKGDQVTIVTA 60 MEHEKDPGWQYLRR+REQ+LEDQSKPYDSKKN WIPDPEEGYLAGEITATKGDQVTIVTA Sbjct 1 MEHEKDPGWQYLRRSREQILEDQSKPYDSKKNCWIPDPEEGYLAGEITATKGDQVTIVTA 60 My questions are; are each of the letters codons of amino acids? In terms of its relationship to eachother and to my initial protein query, what is query 1 and subject 1? I'll deeply appreciate it if you could provide further insights into this database. (maybe there's a crashcourse or something on it somewhere?) My professor seems to be skimming ont he surface of the importance of these databases (blast/wormbase/flybase), but I want to know more about it since there is a good probability that I might find myself using it in the future. Many thanks in advance!