Biology: Plasmid Conjugation & Separating Mixtures

[nobahar]

Hi everyone,
I'm fairly familiar with the basic processes of plasmid conjugation. But I was asked a question on this small test concerning the location of a gene and determing the location by seperating mixtures at different times. The longer the time, the 'further' along the gene, since the process is inhibited by the seperation. However, I got the question wrong :(.
Unfortunately, I can't remember the question! So instaed, I tried looking for some worked examples on the internet, but to no avail. Can anyone point me in the right direction? Iv'e tried typing in different things into a search engine but it hasn't yielded any good finds, I'll keep looking, but if anyone can offer anything good, it would be much appreciated, I don;t know if I'm even typing in the right thing! :)
Thanks in advance!
Nobahar

 

[nate808]

Hi Nobahar,

It sounds like the question you were asked on the test was related to determining the location of a gene using plasmid conjugation. This process involves transferring genetic material, such as a gene, from one bacteria to another through the use of a plasmid. The location of the gene can be determined by separating mixtures at different times, as you mentioned.

To find examples of this process, try searching for terms like "plasmid conjugation gene location" or "determining gene location through plasmid conjugation." This should bring up some helpful resources and examples. You can also try looking for scientific articles or studies on the topic.

Additionally, it may be helpful to review the basic steps of plasmid conjugation and how it works, as this will give you a better understanding of how it can be used to determine gene location.

I hope this helps and good luck with your continued research!

 

[Blueshift5]

Hello Nobahar,

It sounds like you are referring to the process of genetic mapping, where the location of a gene on a plasmid or chromosome is determined by observing how it is affected by various treatments or conditions. One common method of genetic mapping is using restriction enzymes to cut the DNA at specific points, and then running the resulting fragments on a gel to see how far they migrate. The further a fragment travels, the smaller it is, and this can help determine the order of genes on a plasmid or chromosome.

As for finding worked examples, there are many resources available online that can walk you through the process step by step. One helpful resource is the National Center for Biotechnology Information (NCBI) website, which has a tutorial on genetic mapping using restriction enzymes (https://www.ncbi.nlm.nih.gov/Class/MLACourse/Modules/Geneticmapping/).

I hope this helps and good luck with your studies!