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Bradford Assay

  1. Dec 25, 2014 #1
    1. The problem statement, all variables and given/known data
    Hi, I have one more question about the Bradford Assay. This one involves dilution.

    Given 1ml of protein A, I am to determine its protein concentration (in mg/ml) using Bradford Assay (with known BSA protein standards).
    Also, Under a 5 times dilution, the average absorbance is 0.497.

    2. Relevant equations
    The standard curve I obtained is:
    upload_2014-12-26_10-35-0.png

    3. The attempt at a solution
    From the standard curve,
    y = 66.717x
    With absorbance = 0.497
    0.497 = 66.717x
    x = 0.00744 ug/ul
    Since there is a dilution factor of 5
    New x = 0.00744 * 5 = 0.0372 ug/ul = 0.0372 mg/ml ?? (is this correct?)

    However, the correct answer is 0.372 mg/ml . What did I did wrongly??
     
  2. jcsd
  3. Dec 25, 2014 #2

    Bystander

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    Looks good.
    Looks good.

    Answer keys in textbooks are "edited/proofed" by "slave labor," aka grad students and post-docs. Often, "half" changes are made after proofing --- problem statements change, and answers don't.
     
  4. Dec 26, 2014 #3

    Borek

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    In your other question you clearly ignored part of the information given, hard to comment on this one not seeing the whole problem.
     
  5. Dec 26, 2014 #4

    Bystander

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    Is it another part of the same problem? Might be best in future to get all the questions together before asking for help.
     
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