1. The problem statement, all variables and given/known data Hi, I have one more question about the Bradford Assay. This one involves dilution. Given 1ml of protein A, I am to determine its protein concentration (in mg/ml) using Bradford Assay (with known BSA protein standards). Also, Under a 5 times dilution, the average absorbance is 0.497. 2. Relevant equations The standard curve I obtained is: 3. The attempt at a solution From the standard curve, y = 66.717x With absorbance = 0.497 0.497 = 66.717x x = 0.00744 ug/ul Since there is a dilution factor of 5 New x = 0.00744 * 5 = 0.0372 ug/ul = 0.0372 mg/ml ?? (is this correct?) However, the correct answer is 0.372 mg/ml . What did I did wrongly??