- #1

tvtokyo

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## Homework Statement

Hi, I have one more question about the Bradford Assay. This one involves dilution.

Given 1ml of protein A, I am to determine its protein concentration

**(in mg/ml)**using Bradford Assay (with known BSA protein standards).

**Also, Under a 5 times dilution, the average absorbance is 0.497.**

## Homework Equations

The standard curve I obtained is:

## The Attempt at a Solution

From the standard curve,

y = 66.717x

With absorbance = 0.497

0.497 = 66.717x

x = 0.00744

Since there is a dilution factor of 5

New x = 0.00744 * 5 = 0.0372

__ug/ul__Since there is a dilution factor of 5

New x = 0.00744 * 5 = 0.0372

__ug/ul__= 0.0372__mg/ml__?? (is this correct?)However, the correct answer is 0.372

**. What did I did wrongly??**

__mg/ml__