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Buffer help.

  1. Jun 28, 2005 #1
    Would appreciate some assistance from some chem gurus :smile:

    Trying to make the calculations necessary to prepare 0.1 M Tris-HCl buffer. I have 80mL of a 0.1 M tris solution and need to find the volume of 1 N HCl required to adjust the pH of Tris to 8.0. I believe I need to use the Henderson-Hasselbach equation but not sure how to set it up.

    Thank you!
     
  2. jcsd
  3. Jun 29, 2005 #2

    Borek

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    Staff: Mentor

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