Say you are given the velocity of a reaction catalyzed by an enzyme and associated concentrations of substrate, but there is also a competitive inhibitor present. To find Vmax and Km for the reaction you would plot the reciprocal plot of the Mekelis(sp?)-Menton Equation and estimate the Y and X intercepts. My question is, I know that if you plot the slope of the reciprocal plots of the MM equation vs. different concentrations of inhibitor you can find the Kis value for the enzyme inhibitor complex from the x intercept of this plot. Is the Kis value = -(the x intercept) or is -(1/Kis)=x intercept?(adsbygoogle = window.adsbygoogle || []).push({});

**Physics Forums | Science Articles, Homework Help, Discussion**

Dismiss Notice

Join Physics Forums Today!

The friendliest, high quality science and math community on the planet! Everyone who loves science is here!

The friendliest, high quality science and math community on the planet! Everyone who loves science is here!

# Calculating a Kis value for a competitive inhibitor

Can you offer guidance or do you also need help?

Draft saved
Draft deleted

Loading...

Similar Threads for Calculating value competitive |
---|

Does Beer-Lambert's law calculate the absorbance of a solute |

Standard Temp and Pressure -- old values (pre-1982) still prevalent |

**Physics Forums | Science Articles, Homework Help, Discussion**