Calculating Log(MW) & MW with LINEST

  • Thread starter Biochemgirl2002
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    Biochemistry
  • #1
I have found my LINEST equation for my standard curve, which is
where y=log(MW)
AND x= Rf
y=-1.392047709x+2.324113743

as well, my imageLab gives me:
Relative Front (Rf) = 0.087
error on Rf=0.005

I can calculate my log(MW) by inputing my Rf into x value of the equation
y=-1.392047709(0.087)+2.324113743
y=2.203

and my MW can be found by taking it out of log

MW = 10^(2.203)
MW=159.58Da

Now, I want to find my error on the log(MW) and MW,

i think this two part equation will work, but i am unsure

Screen Shot 2021-12-07 at 11.43.03 AM.png
Screen Shot 2021-12-07 at 11.43.15 AM.png
 

Answers and Replies

  • #2
error on log(MW) = error on Rf * 1.392047709error on MW = 10^(2.203) * error on log(MW)error on log(MW) = 0.005 * 1.392047709error on log(MW) = 0.00695error on MW = 159.58 * 0.00695error on MW = 1.11Da
 

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