Can the Hendersen Hasselbach equation be used for higher pH values?

[rockind78]

I am starting to make the various buffers for my research, but I am a bit rusty with my chemistry. I want to know if hendersen hasselbach equation can be used if the desired pH is higher than the pKA. Specifically, I want to make 1.5 M Tris-HCl buffer (pH 8.8) utilizing Tris base and HCl. I can work the math just fine in the HH, but I am a little unusure about the flexibility in its application. Any help would be greatly appreciated. Thank you.

Dustan

 

[Monique]

Here they say it works for pH 8.0 http://ntri.tamuk.edu/fplc/prep.html

As I understand it Tris is the buffering agent, and with HCl and NaOH you can set the pH to the desired point.

 

[ravenprp]

Yes, the Henderson-Hasselbalch equation can be used for higher pH values. The equation is commonly used to calculate the pH of a buffer solution, regardless of the desired pH value. In fact, the equation can be used for any pH value as long as the pKa of the acid and base components are known. In your case, you can definitely use the Henderson-Hasselbalch equation to calculate the pH of your 1.5 M Tris-HCl buffer with a desired pH of 8.8. Just make sure to use the pKa values for Tris base and HCl in your calculations. I hope this helps and good luck with your research!