Dismiss Notice
Join Physics Forums Today!
The friendliest, high quality science and math community on the planet! Everyone who loves science is here!

CDNA to protein sequence?

  1. Feb 14, 2013 #1
    Has anyone here done this before? If so, kit recommendations and protocol? Any help would be awesome. Thanks!
     
  2. jcsd
  3. Feb 14, 2013 #2

    Ygggdrasil

    User Avatar
    Science Advisor
    Insights Author
    2015 Award

    What exactly do you want to do? Do you have a cDNA sequence and you want to figure out the protein sequence that it codes for?
     
  4. Feb 15, 2013 #3
    Yes exactly. Getting the cDNA is easy, I have experience with that for PCR, but instead of doing PCR, I was wondering how you can use your cDNA to get the protein sequence. My PI said it should be easily doable. I'm not sure how though.
     
  5. Feb 15, 2013 #4

    Ygggdrasil

    User Avatar
    Science Advisor
    Insights Author
    2015 Award

    Once you have the cDNA sequence, you just need to sequence it (send a sample of the cDNA along with the appropriate primers to a DNA sequencing facility/company). You can the just read the protein sequence from the sequence you get (for example, using an online translation tool such as http://www.bioinformatics.org/sms2/translate.html

    Let me know if you want more detail on any of these steps.
     
Know someone interested in this topic? Share this thread via Reddit, Google+, Twitter, or Facebook

Have something to add?



Similar Discussions: CDNA to protein sequence?
  1. Protein Synthesis. (Replies: 6)

  2. Competing proteins (Replies: 4)

Loading...