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CDNA to protein sequence?

  1. Feb 14, 2013 #1
    Has anyone here done this before? If so, kit recommendations and protocol? Any help would be awesome. Thanks!
  2. jcsd
  3. Feb 14, 2013 #2


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    What exactly do you want to do? Do you have a cDNA sequence and you want to figure out the protein sequence that it codes for?
  4. Feb 15, 2013 #3
    Yes exactly. Getting the cDNA is easy, I have experience with that for PCR, but instead of doing PCR, I was wondering how you can use your cDNA to get the protein sequence. My PI said it should be easily doable. I'm not sure how though.
  5. Feb 15, 2013 #4


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    Once you have the cDNA sequence, you just need to sequence it (send a sample of the cDNA along with the appropriate primers to a DNA sequencing facility/company). You can the just read the protein sequence from the sequence you get (for example, using an online translation tool such as http://www.bioinformatics.org/sms2/translate.html

    Let me know if you want more detail on any of these steps.
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