# How do I know the minimum of cells for a specific cell line that give a quick entry into log phase growth? #If medium with serum contains trypsin inhibitor. What should I use to dislodge my cells? #Should I always have HCO3- in the medium and what for? # Should pH always be between 7.0 to 7.4 in the medium? What about the osmolarity? Hope for any ideas. Thanks.