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Hi All,
Having plotted a cell growth curve (fluorescence vs days), I have found that during the lag phase, the fluorescence values decrease. I haven't come across this previously during lectures and can't seem to find any examples online to help explain it. My only thought was that cell death occurred at a higher rate than replication whilst the cells were attaching to the culture flask but the decrease occurs between 1 and 3 days so this doesn't seem like a possible explanation. As a side note, 3T3 cells were being used during the experiment.
I'd appreciate any advice on this or resources that may be useful.
Many Thanks
Having plotted a cell growth curve (fluorescence vs days), I have found that during the lag phase, the fluorescence values decrease. I haven't come across this previously during lectures and can't seem to find any examples online to help explain it. My only thought was that cell death occurred at a higher rate than replication whilst the cells were attaching to the culture flask but the decrease occurs between 1 and 3 days so this doesn't seem like a possible explanation. As a side note, 3T3 cells were being used during the experiment.
I'd appreciate any advice on this or resources that may be useful.
Many Thanks