Dismiss Notice
Join Physics Forums Today!
The friendliest, high quality science and math community on the planet! Everyone who loves science is here!

Choice of negative stains

Tags:
  1. Feb 21, 2015 #1
    I have isolated a large molecular complex whose integrity is very sensitive to increasing ionic strength and thus had to be prepared for EM using low ionic strength solutions. And my goal is image this by electron tomography by collecting a dual tilt series of 120 images. Other experimental constraints are that I lack a cold specimen holder and thus must to this experiment at room temperature. From the possible negative stains that are available generally, which should I choose to prepare my specimen and why?
     
    Last edited: Feb 21, 2015
  2. jcsd
  3. Feb 21, 2015 #2

    Pythagorean

    User Avatar
    Gold Member

    You forgot to change the last "your" to "my". Is this a homework question?
     
  4. Feb 21, 2015 #3
    Yes. But can you help me somehow?
     
  5. Feb 21, 2015 #4

    Pythagorean

    User Avatar
    Gold Member

    No, but someone who would be able to help you would probably appreciate some effort in answering the question on your part.
     
Know someone interested in this topic? Share this thread via Reddit, Google+, Twitter, or Facebook




Similar Discussions: Choice of negative stains
  1. Negative Feedback (Replies: 1)

  2. Antibody Staining (Replies: 6)

Loading...