1. Not finding help here? Sign up for a free 30min tutor trial with Chegg Tutors
    Dismiss Notice
Dismiss Notice
Join Physics Forums Today!
The friendliest, high quality science and math community on the planet! Everyone who loves science is here!

Chromatography using acetone!

  1. Oct 2, 2004 #1
    chromatography....using acetone!

    the protocol:

    Vg = volume not accessable to solvent (volume of the gel matrix)

    Vg cannot be measured directly. However, it should apparant that:
    Vg = Vt - volume accessable to solvent

    We can measure the volume accessable to the solvent, using a small molecule such as acetone which can be easily visualized by a UV detector.

    Pharmacia Superose 6 column - volume accessable to solvent = 19.5 mL (measured with acetone)
    Vg = Vt - 19.5 mL = 24.4 - 19.5 mL = 4.9 mL


    hi guys!
    i just find this calculation very unreasonable, since as we know Vt=Vi+Vg+Vo,
    where Vt =total column volum, Vo= void volum due to EXTREMELY huge substances, Vg= volum of matrix and Vi=volum inside the beads due to EXTREMELY small substances.

    my question is, how come we use acetone (small molecule) to determine both Vo and Vi, since Vg= Vt- (Vo+Vi)?

    hopes for replies!

  2. jcsd
  3. Oct 2, 2004 #2
Know someone interested in this topic? Share this thread via Reddit, Google+, Twitter, or Facebook

Have something to add?

Similar Discussions: Chromatography using acetone!
  1. Acetone volatility (Replies: 4)

  2. Acetone and plexiglass (Replies: 2)

  3. How to dry my acetone (Replies: 1)