Dismiss Notice
Join Physics Forums Today!
The friendliest, high quality science and math community on the planet! Everyone who loves science is here!

Chromatography using acetone!

  1. Oct 2, 2004 #1
    chromatography....using acetone!

    the protocol:

    Vg = volume not accessable to solvent (volume of the gel matrix)

    Vg cannot be measured directly. However, it should apparant that:
    Vg = Vt - volume accessable to solvent

    We can measure the volume accessable to the solvent, using a small molecule such as acetone which can be easily visualized by a UV detector.

    Pharmacia Superose 6 column - volume accessable to solvent = 19.5 mL (measured with acetone)
    Vg = Vt - 19.5 mL = 24.4 - 19.5 mL = 4.9 mL


    hi guys!
    i just find this calculation very unreasonable, since as we know Vt=Vi+Vg+Vo,
    where Vt =total column volum, Vo= void volum due to EXTREMELY huge substances, Vg= volum of matrix and Vi=volum inside the beads due to EXTREMELY small substances.

    my question is, how come we use acetone (small molecule) to determine both Vo and Vi, since Vg= Vt- (Vo+Vi)?

    hopes for replies!

  2. jcsd
  3. Oct 2, 2004 #2
Know someone interested in this topic? Share this thread via Reddit, Google+, Twitter, or Facebook

Have something to add?

Similar Discussions: Chromatography using acetone!
  1. Acetone volatility (Replies: 4)

  2. Acetone and plexiglass (Replies: 2)

  3. How to dry my acetone (Replies: 1)