I have a problem that involves a plasmid DNA. The problem gives a plasmid diagram with the restriction sites and specifically tells me which restriction site the gene of interest would go. However, the restriction site is shown to be overlapping with the AmpR gene. Wouldn't that mean the Ampicillin resistance is deactivated when the restriction enzyme cuts the site? I don't get how the gene is supposed to be amplified in bacteria if the plasmid doesn't have AmpR...
Biology - none
The Attempt at a Solution