I have low counting stats and need to subtract background, account for efficiency, and divide by volume. How do I combine the asymmetrical (Poisson) errors?
I am counting the number of particles in 60 fields of view on a scope. I count three pieces of a filter for a sample and three pieces of a filter for a control. All of my counts in 60 fields of view are <50 and Poisson distributed.
The standard way of testing for significant difference is:I want to eventually test the hypothesis that one sample is is greater than the controls. And if two samples are different from each other. This I am ok with- but I have to show all of my calculations for how I can mathematically prove the values are different.
I have small counts in 60 fields of view on a scope and I was propagating error following Gaussian error propagation- which I now know is wrong. But what do I do with these asymmetrical error bars when I want to know sample (+/- error) minus control (+/- error)?
You can't calculate the probability that the null hypothesis is true.From that number, you can calculate the probability of the null hypothesis being true.
You can't calculate the probability that the null hypothesis is true.