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Goodie
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# About the laminar flow hood; when should i turn off the blowers which circulate the air? After the work or should it be on all the time? Can i have it on at the same time with the UV light?
Goodie said:So again, should i leave the UV light 24/7 when i don't work?
Goodie said:Should i turn the blowers off when i have the UV light on?
Should i have the blowers on when i work?
Should i turn the blowers off when i don't work?
A laminar flow hood is a piece of laboratory equipment used to create a sterile working environment by directing filtered air in a uniform, downward flow. The hood works by drawing in air from the surrounding room, filtering it through a High Efficiency Particulate Air (HEPA) filter, and then blowing it out in a laminar flow pattern. This creates a barrier between the work surface and the surrounding environment, reducing the risk of contamination.
The main sources of contamination in a laminar flow hood are the surrounding environment, the operator, and the materials being used. Airborne particles, such as dust and microorganisms, can enter the hood through gaps in the seals or when the hood is opened. The operator's clothing, hair, and skin can also introduce contaminants, as well as the materials being used, such as culture plates or instruments.
To prevent contamination in a laminar flow hood, it is important to properly clean and maintain the hood, as well as follow good aseptic techniques. This includes regularly disinfecting the hood and work surface, wearing appropriate protective clothing, and minimizing movement and talking in the hood's vicinity. It is also important to work quickly and efficiently to reduce the amount of time the hood is open.
If you suspect contamination in your laminar flow hood, stop working immediately and assess the situation. Check for any visible signs of contamination, such as discoloration or growth on the work surface or materials. If contamination is present, disinfect the hood and surrounding area thoroughly before resuming work. It may also be necessary to replace any contaminated materials and re-clean the hood more thoroughly.
The frequency of HEPA filter replacement depends on the type of hood and how often it is used. In general, the filter should be replaced every 6-12 months or when the airflow starts to decrease significantly. It is important to follow the manufacturer's recommendations for filter replacement and to regularly monitor the airflow to ensure the hood is functioning properly.