Hi guys, I have been out of school for a couple of years and I forgot when is it OK to consider a curve crossing at certain value. Let me explain. I am measuring signal in a titration experiment. The outcome is a typical saturation curve (growth curve), a hyperbola. I am wondering if it is OK to set the curve to pass through [0,0]. I subtract the blank and so theoretically, IMO the signal at 0 should be 0. Can you help me or direct me to a webpage where I can refresh my knowledge on this subject? Thanks.