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Denaturation and Peptide Bonds

  1. Aug 5, 2012 #1

    Qube

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    1. The problem statement, all variables and given/known data

    2. Relevant equations

    Peptide bonds DO NOT BREAK DOWN when proteins/enzymes are denatured. Peptide bonds form the primary structure of proteins and enzymes. All of the textbooks here are my sources:

    https://www.google.com/search?q=pep...cp.r_qf.&fp=111e727753762aa8&biw=1366&bih=677


    3. The attempt at a solution

    Conclusion: the CliffNotes book is flat-out W R O N G.

    Am I correct?
     
  2. jcsd
  3. Aug 6, 2012 #2

    chemisttree

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    It would depend on how far above 104oC one goes and how long it's held there but you are right that it is wrong for temperatures only somewhat above that temperature in a reasonable timeframe.
     
  4. Nov 5, 2013 #3
    You are correct.
    Trust me on this one. I'm doing a PhD in biochemistry.

    Heard of the SDS-PAGE technique?
    It involves heating proteins to 100C for 10 mins, and then running them on a gel.
    I've run many, many gels, and my peptide-bonds have never broken.
    You can make out by just looking at the stained-bands
     
    Last edited: Nov 5, 2013
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