1. The problem statement, all variables and given/known data In a mixture of glucose-1-phosphate and glucose-1,6-diphosphate, how could you determine the amount of each in the mixture? 2. Relevant equations N/A 3. The attempt at a solution I was thinking of using a strong acid to separate the phosphate from the glucose and use Fiske-Subbarow assay to determine the amount of glucose-1-phosphate, but won't this also affect the glucose-1,6-diphosphate? I am having trouble figuring out how to determine the amount of glucose-1,6-diphosphate in the mixture.