1. Limited time only! Sign up for a free 30min personal tutor trial with Chegg Tutors
    Dismiss Notice
Dismiss Notice
Join Physics Forums Today!
The friendliest, high quality science and math community on the planet! Everyone who loves science is here!

Determine optical slice thickness in a CLSM

  1. Dec 12, 2008 #1
    I am trying to figure out the optical slice thickness on a confocal laser scanning microscope with the following:

    Lambda exitation: 514
    Lambda emmision: 548nm
    Pinhole: 2 AU / 222,9 um
    NA: 1,2
    n (refraction index): 1,33 (W)

    I have tried a few equations, but they give quite different results. One of the equations I have used is in this article from Zeiss in the following link:
    http://www.zeiss.com/C1256D18002CC306/0/F99A7F3E8944EEE3C1256E5C0045F68B/$file/45-0029_e.pdf"
    (page 11, equation 4)



    I am going to use this in my Master thesis due soon. Can someone please help me determine the correct way of measuring this?
    I must stress that I am a biologist so "be gentle" with the complex formulas :wink:
     
    Last edited by a moderator: Apr 24, 2017
  2. jcsd
Share this great discussion with others via Reddit, Google+, Twitter, or Facebook

Can you offer guidance or do you also need help?
Draft saved Draft deleted