I am trying to figure out the optical slice thickness on a confocal laser scanning microscope with the following:(adsbygoogle = window.adsbygoogle || []).push({});

Lambda exitation: 514

Lambda emmision: 548nm

Pinhole: 2 AU / 222,9 um

NA: 1,2

n (refraction index): 1,33 (W)

I have tried a few equations, but they give quite different results. One of the equations I have used is in this article from Zeiss in the following link:

http://www.zeiss.com/C1256D18002CC306/0/F99A7F3E8944EEE3C1256E5C0045F68B/$file/45-0029_e.pdf"

(page 11, equation 4)

I am going to use this in my Master thesis due soon. Can someone please help me determine the correct way of measuring this?

I must stress that I am a biologist so "be gentle" with the complex formulas

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# Determine optical slice thickness in a CLSM

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