Dismiss Notice
Join Physics Forums Today!
The friendliest, high quality science and math community on the planet! Everyone who loves science is here!

DNA Ligation

  1. Dec 12, 2004 #1
    Is there anyway by which one can selectively prevent 3' end or 5' end of the DNA from ligation?
  2. jcsd
  3. Dec 15, 2004 #2


    User Avatar
    Staff Emeritus
    Science Advisor
    Gold Member

    End capping DNA, one way is by dephosphorylating the phosphate, with CIP (calf intenstestine phophatase). There are other methods available: search for "end capping dna".
Share this great discussion with others via Reddit, Google+, Twitter, or Facebook