DNA Ligation

  • Thread starter karthik3k
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Main Question or Discussion Point

Is there anyway by which one can selectively prevent 3' end or 5' end of the DNA from ligation?
 

Answers and Replies

  • #2
Monique
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End capping DNA, one way is by dephosphorylating the phosphate, with CIP (calf intenstestine phophatase). There are other methods available: search for "end capping dna".
 

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