Misc. Do it yourself test-tube chelation question

  • Thread starter hitssquad
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I have a question:


I am interested in chelating zinc to a dipeptide called http://www.vrp.com/art/462.asp [Broken] ([itex]\beta[/itex]-alanyl-L-histidine) in order to create zinc-carnosine for my own, personal use. Carnosine is known to chelate easily in vivo. However, there are reports stating that ingesting zinc and carnosine together (but unchelated) does not give the same results as ingesting a chelation of zinc and carnosine (carnosine has been demonstrated safe to ingest up to massive quantities, and zinc is safe up to about 50 mg/diem). From this I gather that carnosine does not chelate well with zinc while in a human stomach, or at least that it doesn't do so rapidly-enough to be as helful as ingesting a prefabricated zinc-carnosine chelate.

What general steps would need to be taken to chelate zinc and carnosine in, say, a home kitchen? Would a special type of zinc be needed?


Thanks for any help.



-Chris
 
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911
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Zinc-carnosine update

hitssquad said:
I am interested in chelating zinc to a dipeptide called http://www.vrp.com/art/462.asp [Broken] ([itex]\beta[/itex]-alanyl-L-histidine) in order to create zinc-carnosine for my own, personal use.
There is no longer any need to do this. Now Foods and Jarrow recently started marketing Lonza's zinc-carnosine chelate over-the-counter in the United States:
http://store.yahoo.com/iherb/pepzin.html [Broken]
 
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loseyourname

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Your link to the yahoo! store doesn't work. What is the benefit of taking a zinc-carnosine chelate as opposed to taking a carnosine supplement without the zinc?
 
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Mechanism of action of zinc-carnosine chelate

loseyourname said:
Your link to the yahoo! store doesn't work.
It doesn't show this?:
iherb.jpg




What is the benefit of taking a zinc-carnosine chelate as opposed to taking a carnosine supplement without the zinc?
One beneficial aspect appears to be its slow dissociation rate which allows it to penetrate more deeply into tissue:

--
Mechanism of action. The slow dissociation rate of L-CAZ in gastric juice due to the polymeric character is essentially important because the prolonged existence in the stomach maintains the healing effect for a long time. Two Japanese research groups [5, 6] found strange specific adherent and penetrative characteristics of L-CAZ to the ulcer lesion by staining zinc with dithizone or Timm solution. We assume that such specific adherence of L-CAZ at the ulcer lesion is attributed to the formation of new chemical bond between zinc and body components, i.e., albumin or other proteins to form mixed ligand complexes. L-Carnosine is considered to still bind with zinc at this stage. These body components oozing from the ulcer site can bind strongly to zinc ion with functional groups such as sulfhydryl or imidazole. Via the stage of the mixed ligand complex, L-carnosine, which has wound healing ability due to the anti-free-radical property [7], is released on the ulcer lesion by complete ligand exchange reaction with a body component capable of forming a complex having a larger stability constant than that of the complex formed from L-carnosine. Simultaneously, zinc, which has a protective effect on membranes, is captured completely by the body component and penetrates into the ulcer to ease inflammation. Such ligand exchange reaction was confirmed by the detection of L-carnosine in supernatant or solution when we mix L-CAZ with various materials such as homogenate taken from stomach of rat, rat serum, albumin, and amino acids with sulfhydryl groups. As the ligand exchange reaction proceeds, insoluble L-CAZ gradually dissolves in water. A simple mixture of L-carnosine and zinc presented less anti-ulceration effect, especially on free-radical induced lesions [8], presumably due to rapid diffusion of L-carnosine and zinc ion in the whole stomach. The stability constant of the complex composed of L-carnosine and zinc [9] is inferred to be suitable for the ligand exchange with body components, giving L-carnosine a characteristic property of an excellent carrier of zinc into the living body. This aspect was also confirmed by a study concerning the absorption of zinc from the intestine, as mentioned below [10].
--
http://protein.bio.msu.su/biokhimiya/contents/v65/full/65070961.htm [Broken]
 

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What is the best known source for zinc carnosine to treat H Pylora?

Thankyou for providinng this website dialog....

Walt
 

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