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Effect of inhibitor on enzyme

  • Thread starter leopard
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  • #1
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2pzy4xh.jpg


I'm asked to calculate Vmax and KM from this, with and without the inhibior, and answer what kind of inhibitor it is.

I have plotted 1/ vs 1/V0 here:

2ekrrz9.jpg


The x-intercept is -1/KM. The value I get is -1/KM=-4E4, so that KM=25E-6. Does this mean that KM=25E-6 M? My book says 20-24 M. Of course, I might have been unaccurate in drawing the graph, but why do I miss by a factor of 1E6?
 

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  • #2
epenguin
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Maybe the book says (20-24)uM (micromolar)?

But at your level I have some news for you, part bad part good.

The bad news is sometimes books are wrong. Contain slips. Misprints. That the author does not pick up - can be quite hard to see when you read you own thing.

The good news is you are sometimes right. :smile:
 
Last edited:
  • #3
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Am I right this time?
 
  • #4
epenguin
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Looks right to me.:approve: Of course I could be wrong.:biggrin:

What is your answer to the other part of the question, type of inhibitor?
 
  • #5
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Noncompetitive.
 
  • #6
epenguin
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