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Effect of pH on AmylaseNeed help

  1. Oct 13, 2007 #1
    Hi guys, i have been set some coursework by school on the investigation of the effect of pH on the enzyme amylase. Im abit lost right now only just started A levels. Can someone suggest how i would carry out this investigation? Equipment and method would be much appreciated! And the results need to be quantitative and not qualitative.
  2. jcsd
  3. Oct 13, 2007 #2
    ahh my bad wrong board
  4. Oct 18, 2007 #3
    well you could use your spit (contains the enzyme) , pieces of bread (substrate) ,lemon juice/vinegar (to provide acidic medium) ,water (ph7.7 preferably distilled water),and an alkaline solution.To test to see if glucose has been produced use an iodine solution.
    Blue-black colour indicates that the result is positive.equipment needed : petri dishs :smile:
    Last edited by a moderator: Oct 18, 2007
  5. Oct 18, 2007 #4
    hmmm...problem with iodine is that it doesnt give accurate enough readings to differenciate between the samples
  6. Oct 18, 2007 #5
    Try using a stronger acid and a stronger base. Remember salivary amylase works best in a range of +/- 6.0 - 8.0 .:smile:
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