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Hi all, I have a question about this exercise:

Which is the best option of pH for electrophoresis separation of two peptides with isoelectric points of 10.3 and 7 respectively? The given options of pH are 3,8 or 10.

I've thought that if pH is more than 7, then, there will be positive charge for the peptide of 10.3 pI, and negative charge for the peptide of 7 pI, but i don't know how to manage th sign of the charges to get the best eletrophoresis separation. Is it better to have both of them with the same charge or the opposite charge?

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Which is the best option of pH for electrophoresis separation of two peptides with isoelectric points of 10.3 and 7 respectively? The given options of pH are 3,8 or 10.

I've thought that if pH is more than 7, then, there will be positive charge for the peptide of 10.3 pI, and negative charge for the peptide of 7 pI, but i don't know how to manage th sign of the charges to get the best eletrophoresis separation. Is it better to have both of them with the same charge or the opposite charge?

<Moved to homework section. No template>

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