1. The problem statement, all variables and given/known data A carbonic anhydrase can generate H+ at a rate constant (Kcat) of 106 s-1. 1) What will be the pH in the solution after 0.1 nM of this carbonic anhydrase was incubated with unlimited substrate (CO2) for 1 min (assume the initial pH = 7.0)? 2) What will be the pH in the above reaction solution (0.1 nM of the carbonic anhydrase incubated with unlimited substrate (CO2) for 1 min) if 100 mM Tris (pKa = 8.0) was included as buffer (assume the initial pH = 7.0)? 2. Relevant equations The Henderson-Hasselbach equation, and maybe something to do with Kcat? I have an idea of how to do part 2, but I think it requires the answer to part 1, which I have no idea where to begin with. 3. The attempt at a solution My instructor is very bad about giving us problems after barely explaining or not explaining the method to solve them. Any help is greatly appreciated.