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Enzyme Kinetics - Problems

  1. Oct 6, 2004 #1
    So, we were given a set of data of (uM) and the Velocity (umol/minute) of an inhibitor and no inhibitor.

    we are supposed to find the values of Vmax and Km in the absence of inhibitor and in the presence.

    do I use 1/Vo = 1/Vmax + Km/Vmax - 1/?

    but what is Km and the intial velocity?

    I'm sure this is fundamental for many, but the help would be appreciated.
     
  2. jcsd
  3. Oct 6, 2004 #2
    [tex]\frac{1}{V{o}}=\frac{1}{V{max}}+\frac{K{m}}{V{max}}-\frac{1}{}[/tex]
     
    Last edited: Oct 6, 2004
  4. Oct 6, 2004 #3
    Using that equation, wouldn't we need to know what either [tex]K{m}[/tex] or [tex]V{max}[/tex] is?
     
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