Enzyme Kinetics - Problems

1. Oct 6, 2004

marksju

So, we were given a set of data of (uM) and the Velocity (umol/minute) of an inhibitor and no inhibitor.

we are supposed to find the values of Vmax and Km in the absence of inhibitor and in the presence.

do I use 1/Vo = 1/Vmax + Km/Vmax - 1/?

but what is Km and the intial velocity?

I'm sure this is fundamental for many, but the help would be appreciated.

2. Oct 6, 2004

marksju

$$\frac{1}{V{o}}=\frac{1}{V{max}}+\frac{K{m}}{V{max}}-\frac{1}{}$$

Last edited: Oct 6, 2004
3. Oct 6, 2004

marksju

Using that equation, wouldn't we need to know what either $$K{m}$$ or $$V{max}$$ is?