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Enzyme Kinetics

  1. Oct 20, 2004 #1
    Hey, I'm a newbie here. :smile: Just wanted to say this is a really great site. Lots of informative stuff!

    Anyway, my midterm is coming up and I'm having trouble grasping enzyme kinetics. I'm familiar with the Michaelis - Menten equation, but I don't know how this equation is applied since we never went through any practice problems in class. For example here is a question.

    An enzyme catalyzes a reaction at a velocity of 20 umoles/min when the concentration of substrate is 0.01M. The Km for this substrate is 1X10^-5M. Assuming that Michaelis-Menten kinetics are followed, what will the reaction velocity be when the concentration of substrate is:



    So for part a:

    Km = 1X10^-5M
    = 1X10^-5M
    Vmax = 20umoles/min????

    and we have to find V-inital?

    or is V-initial = 20umoles/min?

    Help is greatly appreciated!
  2. jcsd
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