Hey, I'm a newbie here. Just wanted to say this is a really great site. Lots of informative stuff!(adsbygoogle = window.adsbygoogle || []).push({});

Anyway, my midterm is coming up and I'm having trouble grasping enzyme kinetics. I'm familiar with the Michaelis - Menten equation, but I don't know how this equation is applied since we never went through any practice problems in class. For example here is a question.

An enzyme catalyzes a reaction at a velocity of 20 umoles/min when the concentration of substrate is 0.01M. The Km for this substrate is 1X10^-5M. Assuming that Michaelis-Menten kinetics are followed, what will the reaction velocity be when the concentration of substrate is:

a)1X10^-5M

b)1X10^6M

So for part a:

Km = 1X10^-5M

= 1X10^-5M

Vmax = 20umoles/min????

and we have to find V-inital?

or is V-initial = 20umoles/min?

Help is greatly appreciated!

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# Enzyme Kinetics

Can you offer guidance or do you also need help?

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