Hey, I'm a newbie here. Just wanted to say this is a really great site. Lots of informative stuff! Anyway, my midterm is coming up and I'm having trouble grasping enzyme kinetics. I'm familiar with the Michaelis - Menten equation, but I don't know how this equation is applied since we never went through any practice problems in class. For example here is a question. An enzyme catalyzes a reaction at a velocity of 20 umoles/min when the concentration of substrate is 0.01M. The Km for this substrate is 1X10^-5M. Assuming that Michaelis-Menten kinetics are followed, what will the reaction velocity be when the concentration of substrate is: a)1X10^-5M b)1X10^6M So for part a: Km = 1X10^-5M = 1X10^-5M Vmax = 20umoles/min???? and we have to find V-inital? or is V-initial = 20umoles/min? Help is greatly appreciated!