I did a practical and am now trying to do the data analysis of it. the prac was the alpha-chymotrypsin catalyzed hydrolysis of 4-nitrophenyl trimethylacetate. I have values for X, Y and B and the lab manual has asked "Using the approximated, simplified expressions for X (equation 2), Y (equation 3) and B (equation 4), determine Kcat, [E]0act and K2/K for each kinetic experiment. From these values, determine Km and p." I have calculated Kcat, [E]0act and K2/K, but cant work out how to determine Km. the simplified equations are - equation 2 is simplified to x ≈ Kcat.[E]0act (if [ S] >>km) equation 3 is simplified to y ≈ [E]0act (if [ S] >>Km and K2>>K3) equation 4 is simplified to B ≈ K2[ S]/K (if [ S]>>Km and K2>>K3) where Kcat = K2K3/(K2+K3) ≈ K3 (if K2>>K3) Km = K3K/(K2+K3) the lab manual suggests "to determine Km, consider how K2/K can be derived from the expressions for Kcat and Km." I'm lost and not sure how to solve for Km. I have spent hours on it and just don't understand what it is I'm supposed to do.