- #1
- 4,219
- 67
Does anyone know how far upstream a promotor can go from a gene in higher eukaryotes??
It is very difficult to define a promotor (which contains regulatory elements for a gene), since it is all non-coding DNA with lots of 'junk' in it..
It is fairly easy to determine the transcription start site (TSS) at the 5'UTR, you just isolate mRNA's and look how far upstream they go, and that way determine the major initiation site..
anything upstream of that would be promotor and I guess that the regulatory elements with the most impact would be directly flanking this TSS?
It is very difficult to define a promotor (which contains regulatory elements for a gene), since it is all non-coding DNA with lots of 'junk' in it..
It is fairly easy to determine the transcription start site (TSS) at the 5'UTR, you just isolate mRNA's and look how far upstream they go, and that way determine the major initiation site..
anything upstream of that would be promotor and I guess that the regulatory elements with the most impact would be directly flanking this TSS?