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False Priming sites (Biology)

  1. Oct 18, 2011 #1
    1. The problem statement, all variables and given/known data
    I am reading an article on PCR reactions and I keep coming across the term "False Priming Sites'. I've googled the term and look it up in my text books, but can't find a good definition. Can someone please help?


    2. Relevant equations



    3. The attempt at a solution
     
  2. jcsd
  3. Oct 18, 2011 #2

    Ygggdrasil

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    It likely refers to sequences of DNA that are very similar to the primer sequence and can bind the primer during the PCR reaction. These sites can cause false positives in PCR-based tests or cause extra bands to show up in a PCR reaction.
     
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