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The data in the excel sheet is the numerical representation of the image of leafs taken through spectral imaging camera. The first column represents the wavelength range and other three are the chlorophyll measurements of leaves form 3 different locations. I would like to find out is, if there is any difference in chlorophyll level in these 3 different places. Could anyone please tell me how can i find the first derivative for the given data?

Thanks in advance

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# First Derivative

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