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Fluorescence chemiluminescence

  1. Aug 12, 2011 #1
    Today in lab we performed two different chemiluminescent reactions - one involving the oxidation of luminol with H2O2 in a CuSO4 solution and the other the oxidation of luminol with H2O2 in a hemoglobin solution.

    My question is, why would there be a difference in the duration of the fluorescence of luminol between CuSO4 and hemoglobin? Why would the hemoglobin solution fluoresce for longer?
     
  2. jcsd
  3. Aug 12, 2011 #2

    DrDu

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    I would guess that CuSO4 is a better catalysator for the reaction than is Hemoglobine, but the quantum yield is higher with hemoglobine than with CuSO4.
     
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