1. The problem statement, all variables and given/known data(adsbygoogle = window.adsbygoogle || []).push({});

calculate the angle between the absorption dipole and the emission dipole of specific blue maleimide ?

limiting polarization was determined to be 0.5 and anisotropy 0.4

Relevant equations

I know from lectures that the angle should in fact be zero because the actual max of limiting polarization is 0.5, but I don't know how to work the formula to get to this answer. (if the limiting polarisation was not 0.5 I don’t think I could find the angle)

The attempt at a solution

formula 1/po - 1/3 = 5/3(2 / 3cos2 ɸ -1)

where po is the limiting polarization (0.5)

2. the problem statement, all variables and given/known data

if we add actin (42KDa) to the solution which will bind profiling (14KDa), estimate the anisotropy of the complex?

Partial specific volume v= 0.74

Degree of hydration hy= 0.3g of water per g of protein

Universal gas constant R= 8.13x107

absolute temperature T= 293 ̊c

Viscosity of the medium η= 0.01 dynes.s/cm2

Life time t= 3.32ns

The attempt at a solution

Using tc = ηM(ν + hy)/RT

= 0.01 x 56kda x (0.74 + 0.3) / 8.13x107 x 293

= 5.106x10-12 s

= 5.106x10-3 ns

Then use ro/r = (1+ t/tc)

= 1+ 3.23/5.106x10-3 ns

= 632.59 ?

I don’t know if any of this is right firstly and then if it is how do I get the anisotropy from it because I don’t know the limiting anisotropy?

**Physics Forums | Science Articles, Homework Help, Discussion**

Dismiss Notice

Join Physics Forums Today!

The friendliest, high quality science and math community on the planet! Everyone who loves science is here!

The friendliest, high quality science and math community on the planet! Everyone who loves science is here!

# Homework Help: Fluorescence Spectroscopy Q?

Can you offer guidance or do you also need help?

Draft saved
Draft deleted

**Physics Forums | Science Articles, Homework Help, Discussion**