Dismiss Notice
Join Physics Forums Today!
The friendliest, high quality science and math community on the planet! Everyone who loves science is here!

FRAP in water

  1. Jul 15, 2015 #1
    Hello everyone,

    I have the following question: I'm doing FRAP in water with a quite small molecule in order to get the diffusion coefficient. I'm quite new to FRAP but I have a feeling that this doesn't work very well. I couldn't find any papers or publications in general about someone doing FRAP in a solution. FRAP seems more like a technique for biological cells. Can anyone confirm this or tell me about papers where they perfom FRAP in solution with low viscosity?

    thanks for your help

    Best regards,
    Lindsay



    edit: also I did some measurements, but it seems like quite the impossible task to do the evaluation of the data, because I need a whole cell ROI and background ROI which isn't possible in a liquid because I have no discrete borders like I would have in a cell.
     
  2. jcsd
  3. Jul 15, 2015 #2

    Ygggdrasil

    User Avatar
    Science Advisor

  4. Jul 15, 2015 #3
    thanks for your answer.

    Basically I can detect a typical FRAP "spectrum" but I don't know if I can evaulate correctly because of the problems mentioned above (no full 'cell' ROI) and also no background is available to measure.
     
  5. Jul 17, 2015 #4

    Ygggdrasil

    User Avatar
    Science Advisor

  6. Jul 19, 2015 #5
    thank you very much, I will take a look
     
Know someone interested in this topic? Share this thread via Reddit, Google+, Twitter, or Facebook




Similar Discussions: FRAP in water
  1. Why Water? (Replies: 31)

  2. Water content (Replies: 3)

  3. Water and Plants (Replies: 9)

  4. Water in Biology (Replies: 18)

Loading...