I am looking for some advice on the experiment described below. I am attempting to use electric potential to move negatively charged RNA toward a positive electrode. The procedure is similar to traditional electrophoresis except there is no gel or dies. I attempt to describe the setup below. A transparent 8mm diameter by 20 cm long transparent PVC pipe with a L shaped attachment on either end. Filled with about 15 mL of pH 8 buffer cocktail. Platinum wires are used as electrodes at either end of the tube and the DC power supply ranges from 25-200 Volts. Procedure: I pipette in .07 mg of trulia yeast RNA next to the negative electrode and apply a voltage. After some time, I pipette out 1 mL of fluid (buffer solution with some RNA) from the positive electrode and look for a 260 nm peak in a UV spectrometer. For a control, I repeat the experiment however do not apply a voltage. I have tried the experiment at voltages ranging from 25 - 200 Volts (200 volts melted my tube) I have varied the waiting time from 5 minutes to 30 minutes. I have varied the buffer concentration Thus far I have not yet seen substantially more RNA at the positive electrode then I get in the control. My original theory was the voltage should push almost all the RNA to the positive electrode but that does not seem to be happening. I have noticed that the absorbance peak of the RNA changes substantially when it is sampled near the positive electrode. The peak drops by as much as 15 nm and the shape changes. Any suggestions?