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Diffusion coefficient is what I wanna calculate.

t=w^2*f/(4D)

t:halftime of recovery fluorescence

w:radius of the focused circular laser beam at the e^(-2) intensity

f : correction factor for the amount of bleaching

D:diffusion coefficient

My question is how I can find w.

I know this equation.

I=2P/(pi*w^2)*exp(-(2r^2/w^2))

I:Gaussian intensity profile

For a circular disc profile, I=P/(pi*w^2)

But I don't know exactly how to calculate w.

Does anybody know this?? Please~~.