Molecular cloning experiments are carried out to generate multiple copies of a gene of interest. In one such experiment carried out at a student lab, the gene coding for insulin is multiplied in a bacterial cell. In the screening step, the students realized that they have committed a mistake and they observe many transformed and non-transformed bacterial colonies. Explain this situation with appropriate reasoning.
The Attempt at a Solution
After the restriction enzymes are used, is it that some plastid is not able to recombine with particular gene and the same plastid combine?