Gene insertion

  • Thread starter biophysics
  • Start date
  • #1
26
0

Main Question or Discussion Point

If one gene is inserted into the sequence of a second gene, will both genes by transcribed in the eukaryotic cell?
 

Answers and Replies

  • #2
166
1
Yes, most likely, the whole sequence would be transcribed... as long as the tail of the inserted gene was cut off so that the polymerase would continue to read through it.

Although the translation would probably result in a non functioning protein, and if it was inserted into and intronic region it would be cut out in splicing.
 
  • #3
Monique
Staff Emeritus
Science Advisor
Gold Member
4,149
64
Yes, that is how fusion proteins are made (fluorescently tagged, for instance). Ofcourse it needs to be inserted in frame and there is always a chance that the fusion results in a non-functional protein.
 

Related Threads on Gene insertion

  • Last Post
Replies
5
Views
2K
Replies
2
Views
346
  • Last Post
Replies
2
Views
4K
  • Last Post
Replies
4
Views
7K
  • Last Post
Replies
11
Views
3K
  • Last Post
Replies
3
Views
3K
  • Last Post
Replies
2
Views
2K
  • Last Post
Replies
15
Views
7K
  • Last Post
Replies
2
Views
4K
Top