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Genetics Assignment

  1. Feb 5, 2012 #1
    1. The problem statement, all variables and given/known data
    You decide to revert an iob- mutant to wild type (IOB+) using different mutagens and find the following reversion rates for each mutagen tested:
    2-amino purine - 98%
    5-bromouracil - 95%
    Nitrous acid - 94%
    Proflavin - 2%
    Spontaneous - 4%

    1. What is the most likely molecular basis of the original iob- mutation?
    2. What does the reversion test data suggest about the most prominent mechanism of spontaneous mutations in this organism?
    3. You decide to isolate deletion mutations in the IOB gene. Suggest a genetic screen that would enrich for deletion mutations in this gene over other types of loss-of-function mutations.
    4. Suggest two genetic tests that would show you have indeed identified iob deletion mutants.

    2. Relevant equations



    3. The attempt at a solution
    1. I assumed that since bromouracil, nitrous acid, and aminopurine all cause transition mutations, the original mutation is likely a transition - but i wasn't sure if that's what they meant by molecular basis?
    2. Since I'm assuming the original mutation was a transition, and the rate of spontaneous revertants is very low, I was thinking I'd suggest that the most prominent mechanism of mutations in this organism is not transition mutations - but again i wasn't sure if i was answering what the question was asking
    3. I'm at a loss here - any ideas?! *** I was thinking possibly using a mutagen that is known to cause deletions (but I couldn't seem to find any) or one that causes both deletions and insertions and then screening for insertions by the methods described in 4, but I couldn't seem to find a mutagen like that either. HEEEELP!
    4. If I could answer number 3, then i was thinking i could use PCR analysis or comparative genomic hybridization, does that make sense?

    Your opinions would be much appreciated!
     
  2. jcsd
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