Glow in the dark E.Coli

125
2
Would the methods used to create glow in the dark e.coli readily work on other bacteria?
 
125
2
Ok I guess I'll be more specific.What I mean is will the high school lab experiment method of freezing them and then heat shocking them readily work on other bacteria. Sorry for the unspecific first question I was tired and worded it vaguely.
 
125
2
125
2
I've done it myself in high school.
 
125
2
Yep same procedure will it readily transfer to other bacteria?
 

Ryan_m_b

Staff Emeritus
Science Advisor
5,754
706
GFP (and its derivatives of different colours) has been successfully expressed by multiple species of bacteria. It's even been used in fish and mammals.
 
125
2
Do you know where the derivative Dolores can be purchased? I want to take pond scum from a local lake and make a glowing variety for a freshwater biome
 
125
2
In a fish tank
 

Ryan_m_b

Staff Emeritus
Science Advisor
5,754
706
Please provide a link to "Dolores" so we can know exactly what it is you are asking. Note that GFP shines in UV light so you'll have to have a UV lamp to see anything.
 
125
2
Uggg stupid autocorrect. Sorry for the hassle I meant colors.
 

Ygggdrasil

Science Advisor
Insights Author
Gold Member
2,932
2,093
Do you know where the derivative Dolores can be purchased? I want to take pond scum from a local lake and make a glowing variety for a freshwater biome
Note that most of the organisms commonly referred to as pond scum are not bacteria, but algae, a diverse group of unicellular eukaryotic organisms. While it is certainly possible to introduce fluorescent transgenes into these organisms, the procedures may be different from those used to modify bacteria. However, because there has been considerable research into modifying algae for biofuel applications, there may be some published, standard procedures for introducing transgenes into algae.
 
125
2
Note that most of the organisms commonly referred to as pond scum are not bacteria, but algae, a diverse group of unicellular eukaryotic organisms. While it is certainly possible to introduce fluorescent transgenes into these organisms, the procedures may be different from those used to modify bacteria. However, because there has been considerable research into modifying algae for biofuel applications, there may be some published, standard procedures for introducing transgenes into algae.

Cool thanks, are you by any chance on the bungie forums?
 

Simon Bridge

Science Advisor
Homework Helper
17,823
1,637
Here's the procedure http://www.apsnet.org/edcenter/K-12/...iBacterium.pdf [Broken]
From your reference:
In this lab, Green Fluorescent Protein (GFP) from the bioluminescent jellyfish Aequorea victoria has been incorporated into a plasmid along with a gene for resistance to the antibiotic ampicillin...​
... which is the procedure referred to in the New Scientist article in post #2 isn't it?

Short answer "yes".
 
Last edited by a moderator:
125
2
Kk,.
 

Related Threads for: Glow in the dark E.Coli

  • Last Post
Replies
2
Views
6K
  • Last Post
Replies
14
Views
11K
  • Last Post
Replies
1
Views
4K
Replies
5
Views
3K
Replies
2
Views
1K
Replies
3
Views
3K
Replies
3
Views
505
  • Last Post
Replies
5
Views
3K

Hot Threads

Top