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GRAF123

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## Homework Statement

You are provided with the graph below (attached) about a very interesting microorganism that

produces a new biofuel. From these data, obtained in steady state chemostat (continuous)

cultures for different dilution rates (D, in h-1), limiting substrate (S) and biomass (X)

concentrations:

a) Determine the washout dilution rate;

b) Determine the feed substrate concentration;

c) Determine the cell to substrate yield coefficient (Y_X/S);

d) Determine this microorganism growth kinetic law and estimate the parameters of that relation;

e) Assuming that the production rate of the biofuel expressed by this microorganism obey a Luedeking-Piret law:

q_p= αμ+β

where q_p is the specific production rate of the fuel (g product · g microorganism-1 · h-1) and μ is the specific growth rate of the microorganism (h-1), with α = 0,2 (g product / g microorganism) and β = 0,02 g product · g microorganism-1 · d-1. Identify the dilution rate at which maximum volumetric productivity will be attain and provide the value of that productivity.

## Homework Equations

Y_X/S = (X1-X0)/(S1-S0)

## The Attempt at a Solution

I believe the answers are the following

a) 2 h-1

b) 20 g/L

c) 0.1

d) No idea

e) No idea