Hemolysis from absorbtion

1. Oct 29, 2015

ChrisVer

How can someone calculate the hemolysis (%) from the absorption at λ wavelength given some concentration in NaCl?

The formula I saw was really weird:
Absorb(most hypotonic solution) = 100 % hemolysis
I canlt understand that formula though... thanks

2. Oct 29, 2015

Staff: Mentor

Whoa. This seems a bit lacking in basic information. First off, hemolysis may be a function solely of NaCl concentration, as well, for some types of cells. What you indicate seems to say that the effect of wavelength of light is changed by NaCl concentration. Uhh, where did you get this? As is, I cannot help you. If you can't tell, I'm totally confused.

I would assume that light induced hemolysis is at least in part dictated by the absorption spectrum of the hemoglobins involved, for example.