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Hendersen Hasselbach

  1. Mar 13, 2004 #1
    I am starting to make the various buffers for my research, but I am a bit rusty with my chemistry. I want to know if hendersen hasselbach equation can be used if the desired pH is higher than the pKA. Specifically, I want to make 1.5 M Tris-HCl buffer (pH 8.8) utilizing Tris base and HCl. I can work the math just fine in the HH, but I am a little unusure about the flexibility in its application. Any help would be greatly appreciated. Thank you.

    Dustan
     
  2. jcsd
  3. Mar 14, 2004 #2

    Monique

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    Here they say it works for pH 8.0 http://ntri.tamuk.edu/fplc/prep.html

    As I understand it Tris is the buffering agent, and with HCl and NaOH you can set the pH to the desired point.
     
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