In acetic acid?? but I find it is difficult, which solvent I could use? thank you very much
hot water works fine. Water at about 100C will solubilize it. It will denature.
Undenatured collagen can be solubilized with citrate buffer at about pH of 3.4.
Thank you very much.
Unfortunately, I can't open the link which you give. Is that a paper?? can you tell me the name of the title and the author.
Thank you again
Journal of Applied Polymer Science
Volume 13, Issue 1, Date: January 1969, Pages: 141-148
Leo D. Kahn, Lee P. Witnauer
An automated collagen solubilizing apparatus that maintains constant electrolytic conditions during solubilization is described. It was used in solubilization of 6.95% of a calfskin corium as monomeric collagen in citrate buffer at pH 3.44 and ionic strength of 0.44 and a temperature of 8°C. It is shown that this is the limit of solubilization for undenatured collagen in this buffer.
I have got the paper
Thank you very much
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