Dismiss Notice
Join Physics Forums Today!
The friendliest, high quality science and math community on the planet! Everyone who loves science is here!

How important is composition of TBST in diluting antibodies and Western Blotting?

  1. May 22, 2012 #1


    User Avatar

    I fear that I've added the wrong ingredient in making TBST and I'm not sure what the results will be like for my lab mates. Unfortunately I made 1M Tris-HCl instead of 10x TBS to add to the TBST. In other words, the pH is fine, but I have two times the amount of tris base added and none of the salt. (this is where it gets tricky) However 1L of my 1M Tris-HCl was added to 400mL of a previously fine 10x TBS. Of which I added 800mL to make 8L of TBST which was added to about 3L of stock TBST.

    Basically, how badly did I mess up? Will it simply require more washing? Will it not work at all?
    Any input would be greatly appreciated.

  2. jcsd
Share this great discussion with others via Reddit, Google+, Twitter, or Facebook

Can you offer guidance or do you also need help?
Draft saved Draft deleted