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How to calculate kinetics values?
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[QUOTE="epenguin, post: 6075938, member: 106258"] We often require a student to make more effort to solve a problem. Here we need more effort to tell us what the problem is! We are not told what GOD is, I guess it is nothing transcendental but glucose oxidasae or something. Maybe this dk[SUP]-1[/SUP] is just a misprint? For dt[SUP]-1[/SUP] or something. V[SUB]max[/SUB] Is just the rate of oxidation when the substrate concentration is high (Saturating) - in whatever the units are. The μ mole/sec/mg protein might possibly be a useful in practical work, but is pretty boring.. More interesting would be to get the Turnover number, the number of catalytic events per second per active site, for which you need to know the molecular mass and number of active sites of the enzymes . The K[SUB]m[/SUB] etc presumably refer to glucose But there are two cosubstrates of an oxidase and presumably it refers to conditions of constant, solution-saturating, oxygen concentration. [/QUOTE]
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How to calculate kinetics values?
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