How to create a Lineweaver Burk plot?

[cdoss]

How do you create a Lineweaver Burk plot? I am not quite completely lost, but I am pretty close. I have a bunch of data (absorbances) from an enyme lab that we did and we are supposed to create a Lineweaver Burk Plot. I've tried looking this up on google, but it's all pretty confusing. Can someone explain how to create one in simpler terms?


I have the velocities calculated out (at least I am pretty sure that they are the velocities, I found the slope of the best fit line of the absorbance levels of the enzyme solutions over 120 seconds)

Velocities:
Tube 1: 0.0015
Tube 2: 0.0033
Tube 3: 0.0062
Tube 4: 0.0078
Tube 5: 0.0057

 

[Asim Riaz]

Tube 6: 0.0045To create a Lineweaver Burk Plot, you will need to calculate the reciprocal of each velocity (1/V). Then, plot the reciprocal of the velocities on the x-axis and the 1/ (the inverse of the substrate concentration) on the y-axis. The reciprocal of the velocities are:Tube 1: 666.666 Tube 2: 303.030 Tube 3: 161.290 Tube 4: 127.564 Tube 5: 175.438 Tube 6: 222.222 Then, calculate the 1/, which is the inverse of the substrate concentration. This can be done by taking the amount of substrate in each tube and dividing it by the total volume of the tube. For example, in Tube 1 with a total volume of 10 mL and a substrate concentration of 0.2 mmol, the 1/ would be 0.02/10 = 0.002. Once the 1/[V] and 1/ values are calculated, plot them on the graph. The Lineweaver Burk Plot should be linear, and the slope of the line can be used to determine the Km and Vmax values for the enzyme.