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How to find the concentration of nucleic acid solution using optical density?

  1. May 27, 2012 #1
    I just did this lab where I measured the absorbance of nucleic acid solutions and I have to find the concentration (in mg/mL) of each nucleic acid at different temperatures at 260nm. It is given that they have an absorption maximum at 260nm with an intensity of 17 OD (optical density) units/mg. We are expected to use this value to find the concentrations. I do not know how to go about this with the calculations.

    Here is how I think it should be calculated

    Assuming the measured absorbance was 0.278 and the total volume was 3.05mL

    0.127*1mg/17=7.47*10^-3mg
    7.47*10^-3mg/3.05mL=2.44*10^-3 mg/mL

    Are my calculations right?

    Thank you!
     
  2. jcsd
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