How to get haploid yeast growth (1 Viewer)

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Monique

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Yeast (or only some strains?) can grow as an haploid or a diploid organism.

1. Why can it do both?
2. How do we induce it to grow in a haploid state?
 

iansmith

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Originally posted by Monique
1. Why can it do both?
Because the haploid is the resistant version and least energy demanding and can form spore. Haploids can fuse and create a diploid. thus it increase the genetic diversity.

Originally posted by Monique
2. How do we induce it to grow in a haploid state?
From the life cycle, you grow then in a nutrient poor enviromnent.
 

Monique

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Re: Re: how to get haploid yeast growth

Originally posted by iansmith
Because the haploid is the resistant version and least energy demanding and can form spore. Haploids can fuse and create a diploid. thus it increase the genetic diversity.
Really, I would have expected it the other way around.. haploids only carry one copy of a gene and thus have a high chance of being deficient in biochemical pathways, right?

From the life cycle, you grow then in a nutrient poor enviromnent.
Do you know which nutrient is especially important, or doesn't it really matter?

Thanks, I am just being curious


btw, I heard from a researcher that from the 6000 ORFs in Saccheromyces cerevisea, 2000 are orthologous to humans.. not sure if I trust that person, but it is an indication..
 

iansmith

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Re: Re: Re: how to get haploid yeast growth

Originally posted by Monique
Really, I would have expected it the other way around.. haploids only carry one copy of a gene and thus have a high chance of being deficient in biochemical pathways, right?
Yeah but 1 diploid gives 4 haploids thus increasing the chance of survival. Haploid require less energy to finish their life cycle and they can form spore. The haploid and sporulation mechanism must be regulated by same elements.


Originally posted by Monique
Do you know which nutrient is especially important, or doesn't it really matter?
It migth be the basic requirement. In lab, you could use define minimal medium.

Originally posted by Monique
btw, I heard from a researcher that from the 6000 ORFs in Saccheromyces cerevisea, 2000 are orthologous to humans.. not sure if I trust that person, but it is an indication..
That sounds about rigth. What I heard is that yeast have 37% orthologous to mammals and 2000 in 6000 is 33%.
 

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