IHC detection of proteins in mitochondrial matrix

  • Thread starter Kglocc
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I'm specifically wondering:
Can proteins in the mitochondrial matrix be detected by immunohistochemistry?

I am familiar with the general techniques required for IHC, and I believe the procedures vary depending on what you're looking for. I do think that IHC can stain within the mito-matrix but there must be some special reagents required to do so right?

I spent some time looking for info on this, and I've seen that proteins in the matrix can be stained.

If someone could please confirm or deny this that would be great. Also, I would be interested in the specific protocols required for it, and general limitations of IHC (ie can nuclear proteins be stained, ). Thanks
 

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